4I6O

Crystal structure of chemically synthesized human anaphylatoxin C3a

Summary for 4I6O

Entry DOI	10.2210/pdb4i6o/pdb
Descriptor	Complement C3, (4S)-2-METHYL-2,4-PENTANEDIOL (3 entities in total)
Functional Keywords	disulfide-rich, alpha-helical bundle, drumstick, complement system, third component of complement (c3), local inflammatory process mediator, smooth muscle contraction induction, vascular permeability increase, mast cell histamine release, c3a receptor, gpcr, extracellular, blood plasma, immune system
Biological source	Homo sapiens (human)
Cellular location	Secreted: P01024
Total number of polymer chains	1
Total formula weight	9232.91
Authors	Wang, C.I.A.,Ghassemian, A.,Collins, B.,Lewis, R.J.,Alewood, P.F.,Durek, T. (deposition date: 2012-11-29, release date: 2013-02-27, Last modification date: 2024-10-09)
Primary citation	Ghassemian, A.,Wang, C.I.,Yau, M.K.,Reid, R.C.,Lewis, R.J.,Fairlie, D.P.,Alewood, P.F.,Durek, T. Efficient chemical synthesis of human complement protein C3a. Chem.Commun.(Camb.), 49:2356-2358, 2013 Cited by PubMed Abstract: We report the total chemical synthesis of human C3a by one-pot native chemical ligation of three unprotected peptide segments, followed by efficient in vitro folding that yielded the anaphylatoxin C3a in high yield and excellent purity. Synthetic C3a was fully active and its crystal structure at 2.1 Å resolution showed 3 helices and a C-terminal turn motif. PubMed: 23407800 DOI: 10.1039/c3cc40537k PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.14 Å)

Structure validation