4I4K

Streptomyces globisporus C-1027 9-membered enediyne conserved protein SgcE6

4I4K の概要

• エントリーDOI: 10.2210/pdb4i4k/pdb
• 関連するPDBエントリー: 4OVM
• 分子名称: uncharacterized protein SgcJ, CITRIC ACID, TETRAETHYLENE GLYCOL, ... (7 entities in total)
• 機能のキーワード: structural genomics, psi-biology, midwest center for structural genomics, mcsg, enzyme discovery for natural product biosynthesis, natpro, alpha-beta sandwich, unknown function
• 由来する生物種: Streptomyces globisporus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31040.28

構造登録者

Kim, Y.,Bigelow, L.,Clancy, S.,Babnigg, J.,Bingman, C.A.,Yennamalli, R.,Lohman, J.R.,Ma, M.,Shen, B.,Phillips Jr., G.N.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG),Enzyme Discovery for Natural Product Biosynthesis (NatPro) (登録日: 2012-11-27, 公開日: 2012-12-12, 最終更新日: 2024-11-27)

主引用文献

Huang, T.,Chang, C.Y.,Lohman, J.R.,Rudolf, J.D.,Kim, Y.,Chang, C.,Yang, D.,Ma, M.,Yan, X.,Crnovcic, I.,Bigelow, L.,Clancy, S.,Bingman, C.A.,Yennamalli, R.M.,Babnigg, G.,Joachimiak, A.,Phillips, G.N.,Shen, B.
Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027.
J Antibiot (Tokyo), 69:731-740, 2016

PubMed Abstract: Comparative analysis of the enediyne biosynthetic gene clusters revealed sets of conserved genes serving as outstanding candidates for the enediyne core. Here we report the crystal structures of SgcJ and its homologue NCS-Orf16, together with gene inactivation and site-directed mutagenesis studies, to gain insight into enediyne core biosynthesis. Gene inactivation in vivo establishes that SgcJ is required for C-1027 production in Streptomyces globisporus. SgcJ and NCS-Orf16 share a common structure with the nuclear transport factor 2-like superfamily of proteins, featuring a putative substrate binding or catalytic active site. Site-directed mutagenesis of the conserved residues lining this site allowed us to propose that SgcJ and its homologues may play a catalytic role in transforming the linear polyene intermediate, along with other enediyne polyketide synthase-associated enzymes, into an enzyme-sequestered enediyne core intermediate. These findings will help formulate hypotheses and design experiments to ascertain the function of SgcJ and its homologues in nine-membered enediyne core biosynthesis.

PubMed: 27406907
DOI: 10.1038/ja.2016.88

実験手法

X-RAY DIFFRACTION (1.7 Å)