4HUR

Crystal structure of streptogramin group A antibiotic acetyltransferase VatA from Staphylococcus aureus in complex with acetyl coenzyme A

4HUR の概要

• エントリーDOI: 10.2210/pdb4hur/pdb
• 関連するPDBエントリー: 4E8L 4HUS
• 分子名称: Virginiamycin A acetyltransferase, ACETYL COENZYME *A, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: structural genomics, antibiotic resistance, center for structural genomics of infectious diseases (csgid), niaid, national institute of allergy and infectious diseases, xenobiotic acyltransferase (xat) family, hexapeptide repeat acyltransferase, streptogramin group a antibiotic acetyltransferase, streptogramin group a antibiotics, streptogramin a, virginiamycin m1, dalfopristin, acetyl coenzyme a, coenzyme a, intracellular, transferase
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 79573.84

構造登録者

Stogios, P.J.,Minasov, G.,Evdokimova, E.,Wawrzak, Z.,Yim, V.,Krishnamoorthy, M.,Di Leo, R.,Courvalin, P.,Savchenko, A.,Anderson, W.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2012-11-03, 公開日: 2012-11-14, 最終更新日: 2023-09-20)

主引用文献

Stogios, P.J.,Kuhn, M.L.,Evdokimova, E.,Courvalin, P.,Anderson, W.F.,Savchenko, A.
Potential for Reduction of Streptogramin A Resistance Revealed by Structural Analysis of Acetyltransferase VatA.
Antimicrob.Agents Chemother., 58:7083-7092, 2014

PubMed Abstract: Combinations of group A and B streptogramins (i.e., dalfopristin and quinupristin) are "last-resort" antibiotics for the treatment of infections caused by Gram-positive pathogens, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. Resistance to streptogramins has arisen via multiple mechanisms, including the deactivation of the group A component by the large family of virginiamycin O-acetyltransferase (Vat) enzymes. Despite the structural elucidation performed for the VatD acetyltransferase, which provided a general molecular framework for activity, a detailed characterization of the essential catalytic and antibiotic substrate-binding determinants in Vat enzymes is still lacking. We have determined the crystal structure of S. aureus VatA in apo, virginiamycin M1- and acetyl-coenzyme A (CoA)-bound forms and provide an extensive mutagenesis and functional analysis of the structural determinants required for catalysis and streptogramin A recognition. Based on an updated genomic survey across the Vat enzyme family, we identified key conserved residues critical for VatA activity that are not part of the O-acetylation catalytic apparatus. Exploiting such constraints of the Vat active site may lead to the development of streptogramin A compounds that evade inactivation by Vat enzymes while retaining binding to their ribosomal target.

PubMed: 25223995
DOI: 10.1128/AAC.03743-14

実験手法

X-RAY DIFFRACTION (2.15 Å)