4HS9

Methanol tolerant mutant of the Proteus mirabilis lipase

4HS9 の概要

• エントリーDOI: 10.2210/pdb4hs9/pdb
• 関連するPDBエントリー: 4GW3 4GXN
• 分子名称: Lipase, CALCIUM ION, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: lipase, hydrolase, alpha/beta hydrolase
• 由来する生物種: Proteus mirabilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34673.42

構造登録者

Korman, T.P. (登録日: 2012-10-29, 公開日: 2013-05-22, 最終更新日: 2024-10-16)

主引用文献

Korman, T.P.,Sahachartsiri, B.,Charbonneau, D.M.,Huang, G.L.,Beauregard, M.,Bowie, J.U.
Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution.
Biotechnol Biofuels, 6:70-70, 2013

PubMed Abstract: Biodiesels are methyl esters of fatty acids that are usually produced by base catalyzed transesterification of triacylglyerol with methanol. Some lipase enzymes are effective catalysts for biodiesel synthesis and have many potential advantages over traditional base or acid catalyzed transesterification. Natural lipases are often rapidly inactivated by the high methanol concentrations used for biodiesel synthesis, however, limiting their practical use. The lipase from Proteus mirabilis is a particularly promising catalyst for biodiesel synthesis as it produces high yields of methyl esters even in the presence of large amounts of water and expresses very well in Escherichia coli. However, since the Proteus mirabilis lipase is only moderately stable and methanol tolerant, these properties need to be improved before the enzyme can be used industrially.

PubMed: 23648063
DOI: 10.1186/1754-6834-6-70

実験手法

X-RAY DIFFRACTION (1.8 Å)