4HOB

The crystal structure of the Zalpha domain from Cyprinid Herpes virus 3

4HOB の概要

• エントリーDOI: 10.2210/pdb4hob/pdb
• 関連するPDBエントリー: 1J75 1OYI 1QBJ
• 分子名称: Putative uncharacterized protein, SULFATE ION (3 entities in total)
• 機能のキーワード: domain swapping, z-dna binding domain, dna and rna binding, dna binding protein
• 由来する生物種: Cyprinid herpesvirus 3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 31972.16

構造登録者

Tome, A.R.,Kus, K.,de Rosa, M.,Paulo, L.M.,Figueiredo, D.,Athanasiadis, A. (登録日: 2012-10-22, 公開日: 2013-09-11, 最終更新日: 2023-11-08)

主引用文献

Tome, A.R.,Kus, K.,Correia, S.,Paulo, L.M.,Zacarias, S.,de Rosa, M.,Figueiredo, D.,Parkhouse, R.M.,Athanasiadis, A.
Crystal structure of a poxvirus-like zalpha domain from cyprinid herpesvirus 3
J.Virol., 87:3998-4004, 2013

PubMed Abstract: Zalpha domains are a subfamily of the winged helix-turn-helix domains sharing the unique ability to recognize CpG repeats in the left-handed Z-DNA conformation. In vertebrates, domains of this family are found exclusively in proteins that detect foreign nucleic acids and activate components of the antiviral interferon response. Moreover, poxviruses encode the Zalpha domain-containing protein E3L, a well-studied and potent inhibitor of interferon response. Here we describe a herpesvirus Zalpha-domain-containing protein (ORF112) from cyprinid herpesvirus 3. We demonstrate that ORF112 also binds CpG repeats in the left-handed conformation, and moreover, its structure at 1.75 Å reveals the Zalpha fold found in ADAR1, DAI, PKZ, and E3L. Unlike other Zalpha domains, however, ORF112 forms a dimer through a unique domain-swapping mechanism. Thus, ORF112 may be considered a new member of the Z-domain family having DNA binding properties similar to those of the poxvirus E3L inhibitor of interferon response.

PubMed: 23365431
DOI: 10.1128/JVI.03116-12

実験手法

X-RAY DIFFRACTION (1.76 Å)