4HJ2

Crystal Structure Analysis of GSTA1-1 in complex with chlorambucil

4HJ2 の概要

• エントリーDOI: 10.2210/pdb4hj2/pdb
• 分子名称: Glutathione S-transferase A1, L-gamma-glutamyl-S-(2-{[4-(3-carboxypropyl)phenyl](2-chloroethyl)amino}ethyl)-L-cysteinylglycine (3 entities in total)
• 機能のキーワード: alpha-beta, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P08263
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51218.88

構造登録者

Karpusas, M.,Chiniadis, L.,Labrou, N.E. (登録日: 2012-10-12, 公開日: 2013-03-27, 最終更新日: 2024-02-28)

主引用文献

Karpusas, M.,Axarli, I.,Chiniadis, L.,Papakyriakou, A.,Bethanis, K.,Scopelitou, K.,Clonis, Y.D.,Labrou, N.E.
The interaction of the chemotherapeutic drug chlorambucil with human glutathione transferase A1-1: kinetic and structural analysis.
Plos One, 8:e56337-e56337, 2013

PubMed Abstract: Glutathione transferases (GSTs) are enzymes that contribute to cellular detoxification by catalysing the nucleophilic attack of glutathione (GSH) on the electrophilic centre of a number of xenobiotic compounds, including several chemotherapeutic drugs. In the present work we investigated the interaction of the chemotherapeutic drug chlorambucil (CBL) with human GSTA1-1 (hGSTA1-1) using kinetic analysis, protein crystallography and molecular dynamics. In the presence of GSH, CBL behaves as an efficient substrate for hGSTA1-1. The rate-limiting step of the catalytic reaction between CBL and GSH is viscosity-dependent and kinetic data suggest that product release is rate-limiting. The crystal structure of the hGSTA1-1/CBL-GSH complex was solved at 2.1 Å resolution by molecular replacement. CBL is bound at the H-site attached to the thiol group of GSH, is partially ordered and exposed to the solvent, making specific interactions with the enzyme. Molecular dynamics simulations based on the crystal structure indicated high mobility of the CBL moiety and stabilization of the C-terminal helix due to the presence of the adduct. In the absence of GSH, CBL is shown to be an alkylating irreversible inhibitor for hGSTA1-1. Inactivation of the enzyme by CBL followed a biphasic pseudo-first-order saturation kinetics with approximately 1 mol of CBL per mol of dimeric enzyme being incorporated. Structural analysis suggested that the modifying residue is Cys112 which is located at the entrance of the H-site. The results are indicative of a structural communication between the subunits on the basis of mutually exclusive modification of Cys112, indicating that the two enzyme active sites are presumably coordinated.

PubMed: 23460799
DOI: 10.1371/journal.pone.0056337

実験手法

X-RAY DIFFRACTION (2.1 Å)