4H3Y

Crystal structure of an asymmetric dimer of a tRNA (guanine-(N(1)-)-methyltransferase from Burkholderia phymatum bound to S-adenosyl homocystein in one half-site

4H3Y の概要

• エントリーDOI: 10.2210/pdb4h3y/pdb
• 関連するPDBエントリー: 4H3Z
• 分子名称: tRNA (guanine-N(1)-)-methyltransferase, S-ADENOSYL-L-HOMOCYSTEINE, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: structural genomics, niaid, national institute of allergy and infectious diseases, seattle structural genomics center for infectious disease, ssgcid, trmd, m1g-methyltransferase, g37 methyltransferase, s-adenosyl-methionine, sam, s-adenosyl-homocysteine, sah, trna modification, transferase, proteobacteria, nitrogen fixation, food pathogen, domain swapped homodimer
• 由来する生物種: Burkholderia phymatum
• 細胞内の位置: Cytoplasm (Potential): B2JF31
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61997.72

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2012-09-14, 公開日: 2012-10-03, 最終更新日: 2023-09-20)

主引用文献

Baugh, L.,Gallagher, L.A.,Patrapuvich, R.,Clifton, M.C.,Gardberg, A.S.,Edwards, T.E.,Armour, B.,Begley, D.W.,Dieterich, S.H.,Dranow, D.M.,Abendroth, J.,Fairman, J.W.,Fox, D.,Staker, B.L.,Phan, I.,Gillespie, A.,Choi, R.,Nakazawa-Hewitt, S.,Nguyen, M.T.,Napuli, A.,Barrett, L.,Buchko, G.W.,Stacy, R.,Myler, P.J.,Stewart, L.J.,Manoil, C.,Van Voorhis, W.C.
Combining functional and structural genomics to sample the essential Burkholderia structome.
Plos One, 8:e53851-e53851, 2013

PubMed Abstract: The genus Burkholderia includes pathogenic gram-negative bacteria that cause melioidosis, glanders, and pulmonary infections of patients with cancer and cystic fibrosis. Drug resistance has made development of new antimicrobials critical. Many approaches to discovering new antimicrobials, such as structure-based drug design and whole cell phenotypic screens followed by lead refinement, require high-resolution structures of proteins essential to the parasite.

PubMed: 23382856
DOI: 10.1371/journal.pone.0053851

実験手法

X-RAY DIFFRACTION (2.5 Å)