4H18

Three dimensional structure of corynomycoloyl tranferase C

4H18 の概要

• エントリーDOI: 10.2210/pdb4h18/pdb
• 分子名称: Cmt1, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: alpha / beta hydrolase, mycoloyltransferase, trehalose o-mycolyltransferase, external membrane, transferase
• 由来する生物種: Corynebacterium glutamicum
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 161488.07

構造登録者

Huc, E.,de Sousa D'Auria, C.,Li de la Sierra-Gallay, I.,Salmeron, C.H.,van Tilbeurgh, H.,Bayan, N.,Houssin, C.H.,Daffe, M.,Tropis, M. (登録日: 2012-09-10, 公開日: 2013-09-25, 最終更新日: 2024-10-16)

主引用文献

Huc, E.,de Sousa-D'Auria, C.,de la Sierra-Gallay, I.L.,Salmeron, C.,van Tilbeurgh, H.,Bayan, N.,Houssin, C.,Daffe, M.,Tropis, M.
Identification of a mycoloyl transferase selectively involved in o-acylation of polypeptides in corynebacteriales.
J.Bacteriol., 195:4121-4128, 2013

PubMed Abstract: We have previously described the posttranslational modification of pore-forming small proteins of Corynebacterium by mycolic acid, a very-long-chain α-alkyl and β-hydroxy fatty acid. Using a combination of chemical analyses and mass spectrometry, we identified the mycoloyl transferase (Myt) that catalyzes the transfer of the fatty acid residue to yield O-acylated polypeptides. Inactivation of corynomycoloyl transferase C (cg0413 [Corynebacterium glutamicum mytC {CgmytC}]), one of the six Cgmyt genes of C. glutamicum, specifically abolished the O-modification of the pore-forming proteins PorA and PorH, which is critical for their biological activity. Expectedly, complementation of the cg0413 mutant with either the wild-type gene or its orthologues from Corynebacterium diphtheriae and Rhodococcus, but not Nocardia, fully restored the O-acylation of the porins. Consistently, the three-dimensional structure of CgMytC showed the presence of a unique loop that is absent from enzymes that transfer mycoloyl residues onto both trehalose and the cell wall arabinogalactan. These data suggest the implication of this structure in the enzyme specificity for protein instead of carbohydrate.

PubMed: 23852866
DOI: 10.1128/JB.00285-13

実験手法

X-RAY DIFFRACTION (1.755 Å)