4GWQ

Structure of the Mediator Head Module from S. cerevisiae in complex with the carboxy-terminal domain (CTD) of RNA Polymerase II Rpb1 subunit

4GWQ の概要

• エントリーDOI: 10.2210/pdb4gwq/pdb
• 関連するPDBエントリー: 2HZM 2HZS 3RJ1
• 分子名称: Mediator of RNA polymerase II transcription subunit 11, Mediator of RNA polymerase II transcription subunit 17, Mediator of RNA polymerase II transcription subunit 8, ... (8 entities in total)
• 機能のキーワード: binding sites, mediator complex, models, molecular, phosphorylation, protein structure, tertiary, protein subunits, rna polymerase ii, saccharomyces cerevisiae, structure-activity relationship, transcription
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: Q99278 P32569 P32570 P32585 P34162 P38782 P04050; Nucleus (Probable): P38304
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 245408.84

構造登録者

Robinson, P.J.J.,Bushnell, D.A.,Trnka, M.J.,Burlingame, A.L.,Kornberg, R.D. (登録日: 2012-09-03, 公開日: 2012-10-31, 最終更新日: 2024-02-28)

主引用文献

Robinson, P.J.,Bushnell, D.A.,Trnka, M.J.,Burlingame, A.L.,Kornberg, R.D.
Structure of the Mediator Head module bound to the carboxy-terminal domain of RNA polymerase II.
Proc.Natl.Acad.Sci.USA, 109:17931-17935, 2012

PubMed Abstract: The X-ray crystal structure of the Head module, one-third of the Mediator of transcriptional regulation, has been determined as a complex with the C-terminal domain (CTD) of RNA polymerase II. The structure reveals multiple points of interaction with an extended conformation of the CTD; it suggests a basis for regulation by phosphorylation of the CTD. Biochemical studies show a requirement for Mediator-CTD interaction for transcription.

PubMed: 23071300
DOI: 10.1073/pnas.1215241109

実験手法

X-RAY DIFFRACTION (4.5 Å)