4GT0

Structure of dengue virus serotype 1 sE containing stem to residue 421

4GT0 の概要

• エントリーDOI: 10.2210/pdb4gt0/pdb
• 関連するPDBエントリー: 1OK8 1URZ 3G7T 4GSX
• 分子名称: Envelope protein E, 2-acetamido-2-deoxy-beta-D-glucopyranose, CADMIUM ION, ... (5 entities in total)
• 機能のキーワード: viral fusion protein, viral protein
• 由来する生物種: Dengue virus 1 (DENV-1)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 96668.29

構造登録者

Klein, D.E.,Choi, J.L.,Harrison, S.C. (登録日: 2012-08-28, 公開日: 2012-12-19, 最終更新日: 2024-10-30)

主引用文献

Klein, D.E.,Choi, J.L.,Harrison, S.C.
Structure of a dengue virus envelope protein late-stage fusion intermediate.
J.Virol., 87:2287-2293, 2013

PubMed Abstract: The final stages of dengue virus fusion are thought to occur when the membrane-proximal stem drives the transmembrane anchor of the viral envelope protein (E) toward the fusion loop, buried in the target cell membrane. Crystal structures of E have lacked this essential stem region. We expressed and crystallized soluble mutant forms of the dengue virus envelope protein (sE) that include portions of the juxtamembrane stem. Their structures represent late-stage fusion intermediates. The proximal part of the stem has both intra- and intermolecular interactions, so the chain "zips up" along the trimer seam. The penultimate interaction we detected involves the conserved residue F402, which has hydrophobic contacts with a conserved surface on domain II. These interactions do not require any larger-scale changes in trimer packing. The techniques for expression and crystallization of sE containing stem reported here may allow further characterization of the final stages of flavivirus fusion.

PubMed: 23236058
DOI: 10.1128/JVI.02957-12

実験手法

X-RAY DIFFRACTION (2.57 Å)