4GPA

High resolution structure of the GluA4 N-terminal domain (NTD)

4GPA の概要

• エントリーDOI: 10.2210/pdb4gpa/pdb
• 関連するPDBエントリー: 3hsy 3o21
• 分子名称: Glutamate receptor 4, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: pbp fold, glutamate receptor, ligand-gated ion channel, ion transport, transmembrane ampa receptor regulating proteins, tarps, cornichons, ckamp44, glycosylation, rna editing, cell membrane, synapse, extracellular space, transport protein
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45617.44

構造登録者

Sukumaran, M.,Greger, I.H. (登録日: 2012-08-20, 公開日: 2012-10-24, 最終更新日: 2020-07-29)

主引用文献

Dutta, A.,Shrivastava, I.H.,Sukumaran, M.,Greger, I.H.,Bahar, I.
Comparative Dynamics of NMDA- and AMPA-Glutamate Receptor N-Terminal Domains.
Structure, 20:1838-1849, 2012

PubMed Abstract: Ionotropic glutamate receptors (iGluRs) harbor two extracellular domains: the membrane-proximal ligand-binding domain (LBD) and the distal N-terminal domain (NTD). These are involved in signal sensing: the LBD binds L-glutamate, which activates the receptor channel. Ligand binding to the NTD modulates channel function in the NMDA receptor subfamily of iGluRs, which has not been observed for the AMPAR subfamily to date. Structural data suggest that AMPAR NTDs are packed into tight dimers and have lost their signaling potential. Here, we assess NTD dynamics from both subfamilies, using a variety of computational tools. We describe the conformational motions that underly NMDAR NTD allosteric signaling. Unexpectedly, AMPAR NTDs are capable of undergoing similar dynamics; although dimerization imposes restrictions, the two subfamilies sample similar, interconvertible conformational subspaces. Finally, we solve the crystal structure of AMPAR GluA4 NTD, and combined with molecular dynamics simulations, we characterize regions pivotal for an as-yet-unexplored dynamic spectrum of AMPAR NTDs.

PubMed: 22959625
DOI: 10.1016/j.str.2012.08.012

実験手法

X-RAY DIFFRACTION (2.25 Å)