4GN3

OBody AM1L10 bound to hen egg-white lysozyme

4GN3 の概要

• エントリーDOI: 10.2210/pdb4gn3/pdb
• 関連するPDBエントリー: 4GLA 4GLV 4GN4 4GN5
• 分子名称: Lysozyme C, OBody AM1L10, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: beta barrel, ob-fold, protein-protein complex, novel scaffold, muraminidase, enzyme inhibition, engineered binding protein, inhibitor, hydrolase-de novo protein complex, hydrolase/de novo protein
• 由来する生物種: Pyrobaculum aerophilum; 詳細
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 243600.85

構造登録者

Steemson, J.D.,Liddament, M.T. (登録日: 2012-08-16, 公開日: 2013-08-21, 最終更新日: 2024-11-27)

主引用文献

Steemson, J.D.,Baake, M.,Rakonjac, J.,Arcus, V.L.,Liddament, M.T.
Tracking Molecular Recognition at the Atomic Level with a New Protein Scaffold Based on the OB-Fold.
Plos One, 9:e86050-e86050, 2014

PubMed Abstract: The OB-fold is a small, versatile single-domain protein binding module that occurs in all forms of life, where it binds protein, carbohydrate, nucleic acid and small-molecule ligands. We have exploited this natural plasticity to engineer a new class of non-immunoglobulin alternatives to antibodies with unique structural and biophysical characteristics. We present here the engineering of the OB-fold anticodon recognition domain from aspartyl tRNA synthetase taken from the thermophile Pyrobaculum aerophilum. For this single-domain scaffold we have coined the term OBody. Starting from a naïve combinatorial library, we engineered an OBody with 3 nM affinity for hen egg-white lysozyme, by optimising the affinity of a naïve OBody 11,700-fold over several affinity maturation steps, using phage display. At each maturation step a crystal structure of the engineered OBody in complex with hen egg-white lysozyme was determined, showing binding elements in atomic detail. These structures have given us an unprecedented insight into the directed evolution of affinity for a single antigen on the molecular scale. The engineered OBodies retain the high thermal stability of the parental OB-fold despite mutation of up to 22% of their residues. They can be expressed in soluble form and also purified from bacteria at high yields. They also lack disulfide bonds. These data demonstrate the potential of OBodies as a new scaffold for the engineering of specific binding reagents and provide a platform for further development of future OBody-based applications.

PubMed: 24465865
DOI: 10.1371/journal.pone.0086050

実験手法

X-RAY DIFFRACTION (1.95 Å)