4GGZ

The structure of bradavidin2-biotin complex

4GGZ の概要

• エントリーDOI: 10.2210/pdb4ggz/pdb
• 関連するPDBエントリー: 2Y32 4GGR 4GGT
• 分子名称: Bradavidin 2, BIOTIN (3 entities in total)
• 機能のキーワード: bradavidin, avidin, oligomeric state, streptavidin, high affinity systems, lipocalin fold, beta barrel, biotin binding protein
• 由来する生物種: Bradyrhizobium japonicum
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 51301.87

構造登録者

Livnah, O.,Meir, A. (登録日: 2012-08-07, 公開日: 2013-06-19, 最終更新日: 2024-11-27)

主引用文献

Leppiniemi, J.,Meir, A.,Kahkonen, N.,Kukkurainen, S.,Maatta, J.A.,Ojanen, M.,Janis, J.,Kulomaa, M.S.,Livnah, O.,Hytonen, V.P.
The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins.
Protein Sci., 22:980-994, 2013

PubMed Abstract: Bradavidin II is a biotin-binding protein from Bradyrhizobium japonicum that resembles chicken avidin and bacterial streptavidin. A biophysical characterization was carried out using dynamic light scattering, native mass spectrometry, differential scanning calorimetry, and isothermal titration calorimetry combined with structural characterization using X-ray crystallography. These observations revealed that bradavidin II differs from canonical homotetrameric avidin protein family members in its quaternary structure. In contrast with the other avidins, bradavidin II appears to have a dynamic (transient) oligomeric state in solution. It is monomeric at low protein concentrations but forms higher oligomeric assemblies at higher concentrations. The crystal structure of bradavidin II revealed an important role for Phe42 in shielding the bound ligand from surrounding water molecules, thus functionally replacing the L7,8 loop essential for tight ligand binding in avidin and streptavidin. This bradavidin II characterization opens new avenues for oligomerization-independent biotin-binding protein development.

PubMed: 23661323
DOI: 10.1002/pro.2281

実験手法

X-RAY DIFFRACTION (1.75 Å)