4GGG

Crystal structure of V66A/L68V CzrA in the Zn(II)bound state.

4GGG の概要

• エントリーDOI: 10.2210/pdb4ggg/pdb
• 関連するPDBエントリー: 1R1U 1R1V 2KJB 2KJC
• 分子名称: Repressor protein, ZINC ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: zn(ii) binding protein, transcriptional regulator, arsr/smtb family of transcriptional regulators, zn(ii) sensing transcriptional regulator, transcription regulator
• 由来する生物種: Staphylococcus aureus subsp. aureus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23868.54

構造登録者

Campanello, G.C.,Ma, Z.,Grossoehme, N.E.,Chakrovorty, D.K.,Guerra, A.J.,Ye, Y.,Dann III, C.E.,Merz Jr., K.M.,Giedroc, D.P. (登録日: 2012-08-06, 公開日: 2013-02-06, 最終更新日: 2023-09-13)

主引用文献

Campanello, G.C.,Ma, Z.,Grossoehme, N.E.,Guerra, A.J.,Ward, B.P.,Dimarchi, R.D.,Ye, Y.,Dann, C.E.,Giedroc, D.P.
Allosteric inhibition of a zinc-sensing transcriptional repressor: insights into the arsenic repressor (ArsR) family.
J.Mol.Biol., 425:1143-1157, 2013

PubMed Abstract: The molecular basis of allosteric regulation remains a subject of intense interest. Staphylococcus aureus CzrA is a member of the ubiquitous arsenic repressor (ArsR) family of bacterial homodimeric metal-sensing proteins and has emerged as a model system for understanding allosteric regulation of operator DNA binding by transition metal ions. Using unnatural amino acid substitution and a standard linkage analysis, we show that a His97' NH(ε2)...O=C His67 quaternary structural hydrogen bond is an energetically significant contributor to the magnitude of the allosteric coupling free energy, ∆Gc. A "cavity" introduced just beneath this hydrogen bond in V66A/L68V CzrA results in a significant reduction in regulation by Zn(II) despite adopting a wild-type global structure and Zn(II) binding and DNA binding affinities only minimally affected from wild type. The energetics of Zn(II) binding and heterotropic coupling free energies (∆Hc, -T∆Sc) of the double mutant are also radically altered and suggest that increased internal dynamics leads to poorer allosteric negative regulation in V66A/L68V CzrA. A statistical coupling analysis of 3000 ArsR proteins reveals a sector that links the DNA-binding determinants and the α5 Zn(II)-sensing sites through V66/L68 in CzrA. We propose that distinct regulatory sites uniquely characteristic of individual ArsR proteins result from evolution of distinct connectivities to this sector, each capable of driving the same biological outcome, transcriptional derepression.

PubMed: 23353829
DOI: 10.1016/j.jmb.2013.01.018

実験手法

X-RAY DIFFRACTION (1.998 Å)