4GFA

N-terminal coiled-coil dimer of C.elegans SAS-6, crystal form A

Summary for 4GFA

• Entry DOI: 10.2210/pdb4gfa/pdb
• Related: 2Y3V 2Y3W 3PYI 3Q0X 3Q0Y 4G79 4GEU 4GEX 4GFC
• Descriptor: Spindle assembly abnormal protein 6, (4S)-2-METHYL-2,4-PENTANEDIOL (2 entities in total)
• Functional Keywords: beta-sandwich, alpha-beta protein, structural protein, cytoplasmic, centriolar, central tube
• Biological source: Caenorhabditis elegans
• Cellular location: Cytoplasm: O62479
• Total number of polymer chains: 4
• Total formula weight: 83814.35

Authors

Erat, M.C.,Vakonakis, I. (deposition date: 2012-08-03, release date: 2013-06-19, Last modification date: 2024-02-28)

Primary citation

Hilbert, M.,Erat, M.C.,Hachet, V.,Guichard, P.,Blank, I.D.,Fluckiger, I.,Slater, L.,Lowe, E.D.,Hatzopoulos, G.N.,Steinmetz, M.O.,Gonczy, P.,Vakonakis, I.
Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry.
Proc.Natl.Acad.Sci.USA, 110:11373-11378, 2013

PubMed Abstract: Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas reinhardtii and Danio rerio was shown to form ninefold symmetric, ring-shaped oligomers in vitro that were similar to the cartwheels observed in vivo during early steps of centriole assembly in most species. Here, we report crystallographic and EM analyses showing that, instead, Caenorhabotis elegans SAS-6 self-assembles into a spiral arrangement. Remarkably, we find that this spiral arrangement is also consistent with ninefold symmetry, suggesting that two distinct SAS-6 oligomerization architectures can direct the same output symmetry. Sequence analysis suggests that SAS-6 spirals are restricted to specific nematodes. This oligomeric arrangement may provide a structural basis for the presence of a central tube instead of a cartwheel during centriole assembly in these species.

PubMed: 23798409
DOI: 10.1073/pnas.1302721110

Experimental method

X-RAY DIFFRACTION (3.55 Å)