4GEQ

Crystal structure of the Spc24-Spc25/Cnn1 binding interface

4GEQ の概要

• エントリーDOI: 10.2210/pdb4geq/pdb
• 分子名称: Kinetochore protein SPC25, Kinetochore protein SPC24, Kinetochore-associated protein CNN1, ... (5 entities in total)
• 機能のキーワード: protein-protein complex, ndc80-binding motif, rwd domain, kinetochore components, nucleus, cell cycle
• 由来する生物種: Saccharomyces cerevisiae S288c (yeast); 詳細
• 細胞内の位置: Nucleus : P40014 Q04477 P43618
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 41465.10

構造登録者

Malvezzi, F.,Litos, G.,Schleiffer, A.,Heuck, A.,Clausen, T.,Westermann, S. (登録日: 2012-08-02, 公開日: 2013-01-30, 最終更新日: 2023-09-13)

主引用文献

Malvezzi, F.,Litos, G.,Schleiffer, A.,Heuck, A.,Mechtler, K.,Clausen, T.,Westermann, S.
A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.
Embo J., 32:409-423, 2013

PubMed Abstract: The Ndc80 complex is the key microtubule-binding element of the kinetochore. In contrast to the well-characterized interaction of Ndc80-Nuf2 heads with microtubules, little is known about how the Spc24-25 heterodimer connects to centromeric chromatin. Here, we present molecular details of Spc24-25 in complex with the histone-fold protein Cnn1/CENP-T illustrating how this connection ultimately links microtubules to chromosomes. The conserved Ndc80 receptor motif of Cnn1 is bound as an α helix in a hydrophobic cleft at the interface between Spc24 and Spc25. Point mutations that disrupt the Ndc80-Cnn1 interaction also abrogate binding to the Mtw1 complex and are lethal in yeast. We identify a Cnn1-related motif in the Dsn1 subunit of the Mtw1 complex, necessary for Ndc80 binding and essential for yeast growth. Replacing this region with the Cnn1 peptide restores viability demonstrating functionality of the Ndc80-binding module in different molecular contexts. Finally, phosphorylation of the Cnn1 N-terminus coordinates the binding of the two competing Ndc80 interaction partners. Together, our data provide structural insights into the modular binding mechanism of the Ndc80 complex to its centromere recruiters.

PubMed: 23334295
DOI: 10.1038/emboj.2012.356

実験手法

X-RAY DIFFRACTION (2.01 Å)