4GDO

Structure of a fragment of the rod domain of plectin

Summary for 4GDO

• Entry DOI: 10.2210/pdb4gdo/pdb
• Descriptor: Plectin (2 entities in total)
• Functional Keywords: coiled-coil, structural protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm, cytoskeleton: Q15149
• Total number of polymer chains: 6
• Total formula weight: 29282.48

Authors

De Pereda, J.M.,Buey, R.M.,Uson, I.,Sammito, M.D.,De Marino, I. (deposition date: 2012-08-01, release date: 2013-09-11, Last modification date: 2024-02-28)

Primary citation

Sammito, M.,Millan, C.,Rodriguez, D.D.,de Ilarduya, I.M.,Meindl, K.,De Marino, I.,Petrillo, G.,Buey, R.M.,de Pereda, J.M.,Zeth, K.,Sheldrick, G.M.,Uson, I.
Exploiting tertiary structure through local folds for crystallographic phasing.
Nat.Methods, 10:1099-1101, 2013

PubMed Abstract: We describe an algorithm for phasing protein crystal X-ray diffraction data that identifies, retrieves, refines and exploits general tertiary structural information from small fragments available in the Protein Data Bank. The algorithm successfully phased, through unspecific molecular replacement combined with density modification, all-helical, mixed alpha-beta, and all-beta protein structures. The method is available as a software implementation: Borges.

PubMed: 24037245
DOI: 10.1038/nmeth.2644

Experimental method

X-RAY DIFFRACTION (1.7 Å)