4GD8
SHV-1 beta-lactamase in complex with penam sulfone SA3-53
Summary for 4GD8
| Entry DOI | 10.2210/pdb4gd8/pdb |
| Related | 3D4F 4GD6 4GDB |
| Descriptor | Beta-lactamase SHV-1, (2S,3R)-4-(2-aminoethylcarbamoyloxy)-2-[(2-methanoylindolizin-3-yl)amino]-3-methyl-3-sulfino-butanoic acid, CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE, ... (4 entities in total) |
| Functional Keywords | class a beta-lactamase, hydrolase, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor |
| Biological source | Klebsiella pneumoniae |
| Total number of polymer chains | 1 |
| Total formula weight | 32703.63 |
| Authors | Pattanaik, P.,van den Akker, F. (deposition date: 2012-07-31, release date: 2013-07-31, Last modification date: 2013-08-07) |
| Primary citation | Ke, W.,Pattanaik, P.,Bethel, C.R.,Sheri, A.,Buynak, J.D.,Bonomo, R.A.,van den Akker, F. Structures of SHV-1 beta-lactamase with penem and penam sulfone inhibitors that form cyclic intermediates stabilized by carbonyl conjugation Plos One, 7:e49035-e49035, 2012 Cited by PubMed Abstract: Bacterial β-lactamase enzymes are in large part responsible for the decreased ability of β-lactam antibiotics to combat infections. The inability to overcome β-lactamase mediated resistance spurred the development of inhibitors with penems and penam sulfones being amongst the most potent and broad spectrum mechanism-based inactivators. These inhibitors form covalent, "suicide-type" inhibitory intermediates that are attached to the catalytic S70 residue. To further probe the details of the mechanism of β-lactamase inhibition by these novel compounds, we determined the crystal structures of SHV-1 bound with penem 1, and penam sulfones SA1-204 and SA3-53. Comparison with each other and with previously determined crystal structures of members of these classes of inhibitors suggests that the final conformation of the covalent adduct can vary greatly amongst the complex structures. In contrast, a common theme of carbonyl conjugation as a mechanism to avoid deacylation emerges despite that the penem and penam sulfone inhibitors form different types of intermediates. The detailed insights gained from this study could be used to further improve new mechanism-based inhibitors of these common class A serine β-lactamases. PubMed: 23145056DOI: 10.1371/journal.pone.0049035 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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