4GBF

Crystal structure of the C-terminal domain of gp131 from bacteriophage phiKZ

4GBF の概要

• エントリーDOI: 10.2210/pdb4gbf/pdb
• 分子名称: PHIKZ131, CHLORIDE ION, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: 7-bladed beta-propeller, possibly participates in binding of the phage to the host cell, at the periphery of the baseplate or in the fiber of bacteriophage phikz, viral protein
• 由来する生物種: Pseudomonas phage phiKZ
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 88192.68

構造登録者

Sycheva, L.V.,Shneider, M.M.,Leiman, P.G. (登録日: 2012-07-27, 公開日: 2012-10-17, 最終更新日: 2024-02-28)

主引用文献

Sycheva, L.V.,Shneider, M.M.,Sykilinda, N.N.,Ivanova, M.A.,Miroshnikov, K.A.,Leiman, P.G.
Crystal structure and location of gp131 in the bacteriophage phiKZ virion.
Virology, 434:257-264, 2012

PubMed Abstract: Pseudomonas phage ϕKZ and its two close relatives ϕPA3 and 201ϕ2-1 are very large bacteriophages that form a separate branch in phage classification because their genomes are very different from the rest of GenBank sequence data. The contractile tail of ϕKZ is built from at least 32 different proteins, but a definitive structural function is assigned to only one of them-the tail sheath protein. Here, we report the crystal structure of the C-terminal domain of another phiKZ tail protein, gene product 131 (gp131C). We show that gp131 is located at the periphery of the baseplate and possibly associates with fibers that emanate from the baseplate. Gp131C is a seven-bladed β-propeller that has a shape of a skewed toroid. A small but highly conserved and negatively charged patch on the surface of gp131C might be important for substrate binding or for interaction with a different tail protein.

PubMed: 23031178
DOI: 10.1016/j.virol.2012.09.001

実験手法

X-RAY DIFFRACTION (1.949 Å)