4G6D

G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex

4G6D の概要

• エントリーDOI: 10.2210/pdb4g6d/pdb
• 分子名称: RNA polymerase sigma factor rpoD, ORF067 (3 entities in total)
• 機能のキーワード: staphylococcus aureus rna polymerase, dna binding protein
• 由来する生物種: Staphylococcus aureus subsp. aureus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31957.44

構造登録者

Darst, S.A.,Osmundson, J.S. (登録日: 2012-07-18, 公開日: 2013-01-23, 最終更新日: 2024-02-28)

主引用文献

Osmundson, J.,Montero-Diez, C.,Westblade, L.F.,Hochschild, A.,Darst, S.A.
Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.
Cell(Cambridge,Mass.), 151:1005-1016, 2012

PubMed Abstract: Phage G1 gp67 is a 23 kDa protein that binds to the Staphylococcus aureus (Sau) RNA polymerase (RNAP) σ(A) subunit and blocks cell growth by inhibiting transcription. We show that gp67 has little to no effect on transcription from most promoters but is a potent inhibitor of ribosomal RNA transcription. A 2.0-Å-resolution crystal structure of the complex between gp67 and Sau σ(A) domain 4 (σ(A)(4)) explains how gp67 joins the RNAP promoter complex through σ(A)(4) without significantly affecting σ(A)(4) function. Our results indicate that gp67 forms a complex with RNAP at most, if not all, σ(A)-dependent promoters, but selectively inhibits promoters that depend on an interaction between upstream DNA and the RNAP α-subunit C-terminal domain (αCTD). Thus, we reveal a promoter-specific transcription inhibition mechanism by which gp67 interacts with the RNAP promoter complex through one subunit (σ(A)), and selectively affects the function of another subunit (αCTD) depending on promoter usage.

PubMed: 23178120
DOI: 10.1016/j.cell.2012.10.034

実験手法

X-RAY DIFFRACTION (1.9971 Å)