4G69

Structure of the Human Discs Large 1 PDZ2 - Adenomatous Polyposis Coli Cytoskeletal Polarity Complex

4G69 の概要

• エントリーDOI: 10.2210/pdb4g69/pdb
• 分子名称: Disks large homolog 1, Adenomatous polyposis coli protein (3 entities in total)
• 機能のキーワード: pdz, maguk, polarity, adenomatous polyposis coli, tetramethylrhodamine, adherens junction, membrane protein-signaling protein complex, membrane protein/signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane ; Peripheral membrane protein : Q12959; Cell junction, adherens junction : P25054
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11807.57

構造登録者

Slep, K.C. (登録日: 2012-07-18, 公開日: 2012-12-12, 最終更新日: 2023-09-13)

主引用文献

Slep, K.C.
Structure of the Human Discs Large 1 PDZ2- Adenomatous Polyposis Coli Cytoskeletal Polarity Complex: Insight into Peptide Engagement and PDZ Clustering.
Plos One, 7:e50097-e50097, 2012

PubMed Abstract: The membrane associated guanylate kinase (MAGUK) family member, human Discs Large 1 (hDlg1) uses a PDZ domain array to interact with the polarity determinant, the Adenomatous Polyposis Coli (APC) microtubule plus end binding protein. The hDLG1-APC complex mediates a dynamic attachment between microtubule plus ends and polarized cortical determinants in epithelial cells, stem cells, and neuronal synapses. Using its multi-domain architecture, hDlg1 both scaffolds and regulates the polarity factors it engages. Molecular details underlying the hDlg1-APC interaction and insight into how the hDlg1 PDZ array may cluster and regulate its binding factors remain to be determined. Here, I present the crystal structure of the hDlg1 PDZ2-APC complex and the molecular determinants that mediate APC binding. The hDlg1 PDZ2-APC complex also provides insight into potential modes of ligand-dependent PDZ domain clustering that may parallel Dlg scaffold regulatory mechanisms. The hDlg1 PDZ2-APC complex presented here represents a core biological complex that bridges polarized cortical determinants with the dynamic microtubule cytoskeleton.

PubMed: 23185543
DOI: 10.1371/journal.pone.0050097

実験手法

X-RAY DIFFRACTION (2 Å)