4G2T

Crystal Structure of Streptomyces sp. SF2575 glycosyltransferase SsfS6, complexed with thymidine diphosphate

4G2T の概要

• エントリーDOI: 10.2210/pdb4g2t/pdb
• 関連するPDBエントリー: 4FZR
• 分子名称: SsfS6, THYMIDINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: structural genomics, psi-biology, protein structure initiative, enzyme discovery for natural product biosynthesis, natpro, glycosyltransferase, transferase
• 由来する生物種: Streptomyces sp. SF2575
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43489.04

構造登録者

Wang, F.,Zhou, M.,Singh, S.,Bingman, C.A.,Thorson, J.S.,Phillips Jr., G.N.,Enzyme Discovery for Natural Product Biosynthesis (NatPro) (登録日: 2012-07-12, 公開日: 2012-07-25, 最終更新日: 2024-10-09)

主引用文献

Wang, F.,Zhou, M.,Singh, S.,Yennamalli, R.M.,Bingman, C.A.,Thorson, J.S.,Phillips, G.N.
Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis.
Proteins, 81:1277-1282, 2013

PubMed Abstract: The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 Å resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.

PubMed: 23526584
DOI: 10.1002/prot.24289

実験手法

X-RAY DIFFRACTION (2.405 Å)