4G1M

Re-refinement of alpha V beta 3 structure

4G1M の概要

• エントリーDOI: 10.2210/pdb4g1m/pdb
• 関連するPDBエントリー: 3IJE 4G1E
• 分子名称: Integrin alpha-V, Integrin beta-3, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• 機能のキーワード: protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 190311.93

構造登録者

Springer, T.A.,Mi, L.,Zhu, J. (登録日: 2012-07-10, 公開日: 2012-12-12, 最終更新日: 2024-11-06)

主引用文献

Dong, X.,Mi, L.Z.,Zhu, J.,Wang, W.,Hu, P.,Luo, B.H.,Springer, T.A.
Alpha V Beta 3 Integrin Crystal Structures and their Functional Implications
Biochemistry, 51:8814-8828, 2012

PubMed Abstract: Many questions about the significance of structural features of integrin α(V)β(3) with respect to its mechanism of activation remain. We have determined and re-refined crystal structures of the α(V)β(3) ectodomain linked to C-terminal coiled coils (α(V)β(3)-AB) and four transmembrane (TM) residues in each subunit (α(V)β(3)-1TM), respectively. The α(V) and β(3) subunits with four and eight extracellular domains, respectively, are bent at knees between the integrin headpiece and lower legs, and the headpiece has the closed, low-affinity conformation. The structures differ in the occupancy of three metal-binding sites in the βI domain. Occupancy appears to be related to the pH of crystallization, rather than to the physiologic regulation of ligand binding at the central, metal ion-dependent adhesion site. No electron density was observed for TM residues and much of the α(V) linker. α(V)β(3)-AB and α(V)β(3)-1TM demonstrate flexibility in the linker between their extracellular and TM domains, rather than the previously proposed rigid linkage. A previously postulated interface between the α(V) and β(3) subunits at their knees was also not supported, because it lacks high-quality density, required rebuilding in α(V)β(3)-1TM, and differed markedly between α(V)β(3)-1TM and α(V)β(3)-AB. Together with the variation in domain-domain orientation within their bent ectodomains between α(V)β(3)-AB and α(V)β(3)-1TM, these findings are compatible with the requirement for large structural changes, such as extension at the knees and headpiece opening, in conveying activation signals between the extracellular ligand-binding site and the cytoplasm.

PubMed: 23106217
DOI: 10.1021/bi300734n

実験手法

X-RAY DIFFRACTION (2.9 Å)