4G09

The crystal structure of the C366S mutant of HDH from Brucella suis in complex with a substituted benzyl ketone

4G09 の概要

• エントリーDOI: 10.2210/pdb4g09/pdb
• 関連するPDBエントリー: 4G07
• 分子名称: Histidinol dehydrogenase, ZINC ION, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: rossmann fold, l-histidinol dehydrogenase, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• 由来する生物種: Brucella suis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47816.73

構造登録者

D'Ambrosio, K.,De Simone, G. (登録日: 2012-07-09, 公開日: 2013-10-02, 最終更新日: 2024-02-28)

主引用文献

D'ambrosio, K.,Lopez, M.,Dathan, N.A.,Ouahrani-Bettache, S.,Kohler, S.,Ascione, G.,Monti, S.M.,Winum, J.Y.,De Simone, G.
Structural basis for the rational design of new anti-Brucella agents: The crystal structure of the C366S mutant of l-histidinol dehydrogenase from Brucella suis.
Biochimie, 97:114-120, 2014

PubMed Abstract: L-Histidinol dehydrogenase from Brucella suis (BsHDH) is an enzyme involved in the histidine biosynthesis pathway which is absent in mammals, thus representing a very interesting target for the development of anti-Brucella agents. In this paper we report the crystallographic structure of a mutated form of BsHDH both in its unbound form and in complex with a nanomolar inhibitor. These studies provide the first structural background for the rational design of potent HDH inhibitors, thus offering new hints for clinical applications.

PubMed: 24140957
DOI: 10.1016/j.biochi.2013.09.028

実験手法

X-RAY DIFFRACTION (1.9 Å)