4FYM

Crystal structure of Plasmodium falciparum orotate phosphoribosyltransferase

4FYM の概要

• エントリーDOI: 10.2210/pdb4fym/pdb
• 分子名称: Orotate phosphoribosyltransferase, SULFATE ION (3 entities in total)
• 機能のキーワード: rossmann fold, transferase
• 由来する生物種: Plasmodium falciparum
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 254582.95

構造登録者

Rathod, P.K.,Kumar, S. (登録日: 2012-07-05, 公開日: 2013-08-07, 最終更新日: 2024-02-28)

主引用文献

Kumar, S.,Krishnamoorthy, K.,Mudeppa, D.G.,Rathod, P.K.
Structure of Plasmodium falciparum orotate phosphoribosyltransferase with autologous inhibitory protein-protein interactions.
Acta Crystallogr F Struct Biol Commun, 71:600-608, 2015

PubMed Abstract: The most severe form of malaria is caused by the obligate parasite Plasmodium falciparum. Orotate phosphoribosyltransferase (OPRTase) is the fifth enzyme in the de novo pyrimidine-synthesis pathway in the parasite, which lacks salvage pathways. Among all of the malaria de novo pyrimidine-biosynthesis enzymes, the structure of P. falciparum OPRTase (PfOPRTase) was the only one unavailable until now. PfOPRTase that could be crystallized was obtained after some low-complexity sequences were removed. Four catalytic dimers were seen in the asymmetic unit (a total of eight polypeptides). In addition to revealing unique amino acids in the PfOPRTase active sites, asymmetric dimers in the larger structure pointed to novel parasite-specific protein-protein interactions that occlude the catalytic active sites. The latter could potentially modulate PfOPRTase activity in parasites and possibly provide new insights for blocking PfOPRTase functions.

PubMed: 25945715
DOI: 10.1107/S2053230X1500549X

実験手法

X-RAY DIFFRACTION (2.6 Å)