4FWX

Aquoferric F33Y CuB myoglobin (F33Y L29H F43H sperm whale myoglobin)

4FWX の概要

• エントリーDOI: 10.2210/pdb4fwx/pdb
• 関連するPDBエントリー: 4FWY 4FWZ
• 分子名称: Myoglobin, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: globin, oxidase, transport protein
• 由来する生物種: Physeter catodon (Sperm whale)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17883.40

構造登録者

Gao, Y.-G.,Stoner-Ma, D.,Robinson, H.,Petrik, I.D.,Miner, K.D.,Lu, Y. (登録日: 2012-07-02, 公開日: 2012-07-18, 最終更新日: 2023-09-13)

主引用文献

Miner, K.D.,Mukherjee, A.,Gao, Y.G.,Null, E.L.,Petrik, I.D.,Zhao, X.,Yeung, N.,Robinson, H.,Lu, Y.
A Designed Functional Metalloenzyme that Reduces O(2) to H(2) O with Over One Thousand Turnovers.
Angew.Chem.Int.Ed.Engl., 51:5589-5592, 2012

PubMed Abstract: [Image: see text] Rational design of functional enzymes with high turnovers is a significant challenge, especially those with complex active site and difficult reactions, such as in respiratory oxidases. Introducing 2 His and 1 Tyr into myoglobin resulted in designed enzymes that reduce O to HO with > 1000 turnovers and minimal release of reactive oxygen species. This also showed that presence and positioning of Tyr, not Cu, are critical for activity.

PubMed: 22539151
DOI: 10.1002/anie.201201981

実験手法

X-RAY DIFFRACTION (1.9 Å)