4FUS

The X-ray structure of Hahella chejuensis family 48 glycosyl hydrolase

4FUS の概要

• エントリーDOI: 10.2210/pdb4fus/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900005
• 分子名称: RTX toxins and related Ca2+-binding protein, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, DI(HYDROXYETHYL)ETHER, ... (10 entities in total)
• 機能のキーワード: glycosyl hydrolase, gh48, hydrolase
• 由来する生物種: Hahella chejuensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 80078.75

構造登録者

Alahuhta, P.M.,Lunin, V.V. (登録日: 2012-06-28, 公開日: 2012-10-17, 最終更新日: 2023-12-06)

主引用文献

Sukharnikov, L.O.,Alahuhta, M.,Brunecky, R.,Upadhyay, A.,Himmel, M.E.,Lunin, V.V.,Zhulin, I.B.
Sequence, structure, and evolution of cellulases in glycoside hydrolase family 48.
J.Biol.Chem., 287:41068-41077, 2012

PubMed Abstract: Currently, the cost of cellulase enzymes remains a key economic impediment to commercialization of biofuels. Enzymes from glycoside hydrolase family 48 (GH48) are a critical component of numerous natural lignocellulose-degrading systems. Although computational mining of large genomic data sets is a promising new approach for identifying novel cellulolytic activities, current computational methods are unable to distinguish between cellulases and enzymes with different substrate specificities that belong to the same protein family. We show that by using a robust computational approach supported by experimental studies, cellulases and non-cellulases can be effectively identified within a given protein family. Phylogenetic analysis of GH48 showed non-monophyletic distribution, an indication of horizontal gene transfer. Enzymatic function of GH48 proteins coded by horizontally transferred genes was verified experimentally, which confirmed that these proteins are cellulases. Computational and structural studies of GH48 enzymes identified structural elements that define cellulases and can be used to computationally distinguish them from non-cellulases. We propose that the structural element that can be used for in silico discrimination between cellulases and non-cellulases belonging to GH48 is an ω-loop located on the surface of the molecule and characterized by highly conserved rare amino acids. These markers were used to screen metagenomics data for "true" cellulases.

PubMed: 23055526
DOI: 10.1074/jbc.M112.405720

実験手法

X-RAY DIFFRACTION (1.75 Å)