4FT8

E. coli Catabolite Activator Protein with Cobalt and Sulfate Ligands

4FT8 の概要

• エントリーDOI: 10.2210/pdb4ft8/pdb
• 分子名称: Catabolite gene activator, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: winged helix-turn-helix, dna binding protein, cobalt binding, sulfate binding, transcription activator
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49107.58

構造登録者

Rao, R.,Lawson, C.L. (登録日: 2012-06-27, 公開日: 2013-12-18, 最終更新日: 2023-09-13)

主引用文献

Rao, R.R.,Lawson, C.L.
Structure of catabolite activator protein with cobalt(II) and sulfate.
Acta Crystallogr F Struct Biol Commun, 70:560-563, 2014

PubMed Abstract: The crystal structure of cyclic AMP-catabolite activator protein (CAP) from Escherichia coli containing cobalt(II) chloride and ammonium sulfate is reported at 1.97 Å resolution. Each of the two CAP subunits in the asymmetric unit binds one cobalt(II) ion, in each case coordinated by N-terminal domain residues His19, His21 and Glu96 plus an additional acidic residue contributed via a crystal contact. The three identified N-terminal domain cobalt-binding residues are part of a region of CAP that is important for transcription activation at class II CAP-dependent promoters. Sulfate anions mediate additional crystal lattice contacts and occupy sites corresponding to DNA backbone phosphate positions in CAP-DNA complex structures.

PubMed: 24817710
DOI: 10.1107/S2053230X14005366

実験手法

X-RAY DIFFRACTION (1.966 Å)