4FS5
Crystal structure of the Z-DNA hexamer CGCGCG at 500 mM MgCl2
Summary for 4FS5
| Entry DOI | 10.2210/pdb4fs5/pdb |
| Related | 4FS6 |
| Descriptor | DNA (5'-D(*CP*GP*CP*GP*CP*G)-3'), MAGNESIUM ION, CHLORIDE ION, ... (4 entities in total) |
| Functional Keywords | z-dna, mg2+ binding, dna |
| Total number of polymer chains | 2 |
| Total formula weight | 4005.27 |
| Authors | Chatake, T.,Sunami, T. (deposition date: 2012-06-26, release date: 2013-05-15, Last modification date: 2023-11-08) |
| Primary citation | Chatake, T.,Sunami, T. Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl2 and CaCl2 J.Inorg.Biochem., 124:15-25, 2013 Cited by PubMed Abstract: In this study, crystals of Z-DNA hexamer d(CGCGCG) complexed with MgCl2 and CaCl2 were obtained in the presence of high concentrations of alkaline earth salts (500mM) using a temperature control technique, and their crystal structures were determined at 1.3Å resolution. Mg(2+) and Ca(2+) cations in these structures tend to interact directly with phosphate groups of Z-DNA duplexes; however, they tend to form water-mediated interactions with Z-DNA in the presence of lower concentrations of alkaline earth salts. In these crystals, a DNA duplex was laid along its c-axis and interacted with its 6 neighboring DNA duplexes through coordination bonds of PO…(Mg(2+) or Ca(2+))…OP. A symmetrical hexagonal Z-DNA duplex assembly model may explain DNA condensation caused by alkaline earth salts. These structures offer insights into the functions of alkaline earth cations essential to the structures and assembly of Z-DNA duplexes. PubMed: 23578858DOI: 10.1016/j.jinorgbio.2013.03.004 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.3 Å) |
Structure validation
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