4FS5

Crystal structure of the Z-DNA hexamer CGCGCG at 500 mM MgCl2

4FS5 の概要

• エントリーDOI: 10.2210/pdb4fs5/pdb
• 関連するPDBエントリー: 4FS6
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3'), MAGNESIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: z-dna, mg2+ binding, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 4005.27

構造登録者

Chatake, T.,Sunami, T. (登録日: 2012-06-26, 公開日: 2013-05-15, 最終更新日: 2023-11-08)

主引用文献

Chatake, T.,Sunami, T.
Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl2 and CaCl2
J.Inorg.Biochem., 124:15-25, 2013

PubMed Abstract: In this study, crystals of Z-DNA hexamer d(CGCGCG) complexed with MgCl2 and CaCl2 were obtained in the presence of high concentrations of alkaline earth salts (500mM) using a temperature control technique, and their crystal structures were determined at 1.3Å resolution. Mg(2+) and Ca(2+) cations in these structures tend to interact directly with phosphate groups of Z-DNA duplexes; however, they tend to form water-mediated interactions with Z-DNA in the presence of lower concentrations of alkaline earth salts. In these crystals, a DNA duplex was laid along its c-axis and interacted with its 6 neighboring DNA duplexes through coordination bonds of PO…(Mg(2+) or Ca(2+))…OP. A symmetrical hexagonal Z-DNA duplex assembly model may explain DNA condensation caused by alkaline earth salts. These structures offer insights into the functions of alkaline earth cations essential to the structures and assembly of Z-DNA duplexes.

PubMed: 23578858
DOI: 10.1016/j.jinorgbio.2013.03.004

実験手法

X-RAY DIFFRACTION (1.3 Å)