4FH1

S. cerevisiae Ubc13-N79A

4FH1 の概要

• エントリーDOI: 10.2210/pdb4fh1/pdb
• 分子名称: Ubiquitin-conjugating enzyme E2 13, 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID (3 entities in total)
• 機能のキーワード: ubiquitin conjugating enzyme, ligase
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17638.18

構造登録者

Berndsen, C.E.,Wolberger, C.,Wiener, R. (登録日: 2012-06-05, 公開日: 2013-01-09, 最終更新日: 2023-09-13)

主引用文献

Berndsen, C.E.,Wiener, R.,Yu, I.W.,Ringel, A.E.,Wolberger, C.
A conserved asparagine has a structural role in ubiquitin-conjugating enzymes.
Nat.Chem.Biol., 9:154-156, 2013

PubMed Abstract: It is widely accepted that ubiquitin-conjugating enzymes contain an active site asparagine that serves as an oxyanion hole, thereby stabilizing a negatively charged transition state intermediate and promoting ubiquitin transfer. Using structural and biochemical approaches to study the role of the conserved asparagine to ubiquitin conjugation by Ubc13-Mms2, we conclude that the importance of this residue stems primarily from its structural role in stabilizing an active site loop.

PubMed: 23292652
DOI: 10.1038/nchembio.1159

実験手法

X-RAY DIFFRACTION (2.61 Å)