4FGW

Structure of Glycerol-3-Phosphate Dehydrogenase, GPD1, from Sacharomyces Cerevisiae

4FGW の概要

• エントリーDOI: 10.2210/pdb4fgw/pdb
• 分子名称: Glycerol-3-phosphate dehydrogenase [NAD(+)] 1 (2 entities in total)
• 機能のキーワード: dehydrogenase, nad+, oxidoreductase
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Cytoplasm: Q00055
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85831.38

構造登録者

Aparicio, D.,Munmun, N.,Carpena, X.,Fita, I.,Loewen, P. (登録日: 2012-06-05, 公開日: 2012-11-07, 最終更新日: 2023-09-13)

主引用文献

Alarcon, D.A.,Nandi, M.,Carpena, X.,Fita, I.,Loewen, P.C.
Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45A resolution
Acta Crystallogr.,Sect.F, 68:1279-1283, 2012

PubMed Abstract: The interconversion of glycerol 3-phosphate and dihydroxyacetone phosphate by glycerol-3-phosphate dehydrogenases provides a link between carbohydrate and lipid metabolism and provides Saccharomyces cerevisiae with protection against osmotic and anoxic stress. The first structure of a glycerol-3-phosphate dehydrogenase from S. cerevisiae, GPD1, is reported at 2.45 Å resolution. The asymmetric unit contains two monomers, each of which is organized with N- and C-terminal domains. The N-terminal domain contains a classic Rossmann fold with the (β-α-β-α-β)2 motif typical of many NAD+-dependent enzymes, while the C-terminal domain is mainly α-helical. Structural and phylogenetic comparisons reveal four main structure types among the five families of glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases and reveal that the Clostridium acetobutylican protein with PDB code 3ce9 is a glycerol-1-phosphate dehydrogenase.

PubMed: 23143232
DOI: 10.1107/S1744309112037736

実験手法

X-RAY DIFFRACTION (2.45 Å)