4FES

Structure of OSH4 in complex with cholesterol analogs

Summary for 4FES

• Entry DOI: 10.2210/pdb4fes/pdb
• Related: 1zhy 4F4B
• Descriptor: Protein KES1, (3S,8S,9S,10R,13S,14S,17R)-3-hydroxy-10,13-dimethyl-17-[(2S,6S)-6-methyl-3-oxooctan-2-yl]-1,2,3,4,7,8,9,10,11,12,13,14,15,17-tetradecahydro-16H-cyclopenta[a]phenanthren-16-one (3 entities in total)
• Functional Keywords: oxysterol, sterol binding protein, lipid binding protein
• Biological source: Saccharomyces cerevisiae (Baker's yeast)
• Cellular location: Golgi apparatus membrane : P35844
• Total number of polymer chains: 2
• Total formula weight: 100397.49

Authors

Koag, M.C.,Monzingo, A.F.,Cheun, Y.,Lee, S. (deposition date: 2012-05-30, release date: 2013-06-12, Last modification date: 2023-09-13)

Primary citation

Koag, M.C.,Cheun, Y.,Kou, Y.,Ouzon-Shubeita, H.,Min, K.,Monzingo, A.F.,Lee, S.
Synthesis and structure of 16,22-diketocholesterol bound to oxysterol-binding protein Osh4.
Steroids, 78:938-944, 2013

PubMed Abstract: We have synthesized 16,22-diketocholesterol, a novel ligand for oxysterol-binding protein Osh4, and determined X-ray structure of the diketocholesterol in complex with Osh4. The X-ray structure shows that α7 helix of Osh4 assumes open conformation while the rest of Osh4, closed conformation, implying this diketocholesterol-bound Osh4 structure may represent a structural intermediate between the two conformations.

PubMed: 23756172
DOI: 10.1016/j.steroids.2013.05.016

Experimental method

X-RAY DIFFRACTION (2 Å)