4FES

Structure of OSH4 in complex with cholesterol analogs

4FES の概要

• エントリーDOI: 10.2210/pdb4fes/pdb
• 関連するPDBエントリー: 1zhy 4F4B
• 分子名称: Protein KES1, (3S,8S,9S,10R,13S,14S,17R)-3-hydroxy-10,13-dimethyl-17-[(2S,6S)-6-methyl-3-oxooctan-2-yl]-1,2,3,4,7,8,9,10,11,12,13,14,15,17-tetradecahydro-16H-cyclopenta[a]phenanthren-16-one (3 entities in total)
• 機能のキーワード: oxysterol, sterol binding protein, lipid binding protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Golgi apparatus membrane : P35844
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 100397.49

構造登録者

Koag, M.C.,Monzingo, A.F.,Cheun, Y.,Lee, S. (登録日: 2012-05-30, 公開日: 2013-06-12, 最終更新日: 2023-09-13)

主引用文献

Koag, M.C.,Cheun, Y.,Kou, Y.,Ouzon-Shubeita, H.,Min, K.,Monzingo, A.F.,Lee, S.
Synthesis and structure of 16,22-diketocholesterol bound to oxysterol-binding protein Osh4.
Steroids, 78:938-944, 2013

PubMed Abstract: We have synthesized 16,22-diketocholesterol, a novel ligand for oxysterol-binding protein Osh4, and determined X-ray structure of the diketocholesterol in complex with Osh4. The X-ray structure shows that α7 helix of Osh4 assumes open conformation while the rest of Osh4, closed conformation, implying this diketocholesterol-bound Osh4 structure may represent a structural intermediate between the two conformations.

PubMed: 23756172
DOI: 10.1016/j.steroids.2013.05.016

実験手法

X-RAY DIFFRACTION (2 Å)