4FEC

Crystal Structure of Htt36Q3H

4FEC の概要

• エントリーDOI: 10.2210/pdb4fec/pdb
• 関連するPDBエントリー: 3IO4 3IO6 3IOR 3IOT 3IOU 3IOV 4FE8 4FEB 4FED
• 分子名称: Maltose-binding periplasmic protein,Huntingtin, ZINC ION (3 entities in total)
• 機能のキーワード: alpha helix, loop, beta-strand hairpin, beta strand hairpin, disease protein, signaling protein
• 由来する生物種: Escherichia coli (strain K12); 詳細
• 細胞内の位置: Cytoplasm: P42858
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 152352.64

構造登録者

Kim, M. (登録日: 2012-05-30, 公開日: 2013-03-13, 最終更新日: 2023-11-29)

主引用文献

Kim, M.
Beta conformation of polyglutamine track revealed by a crystal structure of Huntingtin N-terminal region with insertion of three histidine residues.
Prion, 7:221-228, 2013

PubMed Abstract: Huntington disease is an autosomal-dominant neurodegenerative disorder caused by a polyglutamine (polyQ) expansion (> 35Q) in the first exon (EX1) of huntingtin protein (Htt). mHtt protein is thought to adopt one or more toxic conformation(s) that are involved in pathogenic interactions in cells . However, the structure of mHtt is not known. Here, we present a near atomic resolution structure of mHtt36Q-EX1. To facilitate crystallization, three histidine residues (3H) were introduced within the Htt36Q stretch resulting in the sequence of Q 7HQHQHQ 27. The Htt36Q3H region adopts α-helix, loop, β-hairpin conformations. Furthermore, we observed interactions between the backbone of the Htt36Q3H β-strand with the aromatic residues mimicking putative-toxic interactions with other proteins. Our findings support previous predictions that the expanded mHtt-polyQ region adopts a β-sheet structure. Detailed structural information about mHtt improves our understanding of the pathogenic mechanisms in HD and other polyQ expansion disorders and may form the basis for rational design of small molecules that target toxic conformations of disease-causing proteins.

PubMed: 23370273
DOI: 10.4161/pri.23807

実験手法

X-RAY DIFFRACTION (3 Å)