4FDG

Crystal Structure of an Archaeal MCM Filament

4FDG の概要

• エントリーDOI: 10.2210/pdb4fdg/pdb
• 分子名称: Minichromosome maintenance protein MCM, ZINC ION (2 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Sulfolobus Solfataricus
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 392155.44

構造登録者

Slaymaker, I.M.,Fu, Y.,Brewster, A.B.,Chen, X.S. (登録日: 2012-05-28, 公開日: 2013-03-06, 最終更新日: 2024-02-28)

主引用文献

Slaymaker, I.M.,Fu, Y.,Toso, D.B.,Ranatunga, N.,Brewster, A.,Forsburg, S.L.,Zhou, Z.H.,Chen, X.S.
Mini-chromosome maintenance complexes form a filament to remodel DNA structure and topology.
Nucleic Acids Res., 41:3446-3456, 2013

PubMed Abstract: Deregulation of mini-chromosome maintenance (MCM) proteins is associated with genomic instability and cancer. MCM complexes are recruited to replication origins for genome duplication. Paradoxically, MCM proteins are in excess than the number of origins and are associated with chromatin regions away from the origins during G1 and S phases. Here, we report an unusually wide left-handed filament structure for an archaeal MCM, as determined by X-ray and electron microscopy. The crystal structure reveals that an α-helix bundle formed between two neighboring subunits plays a critical role in filament formation. The filament has a remarkably strong electro-positive surface spiraling along the inner filament channel for DNA binding. We show that this MCM filament binding to DNA causes dramatic DNA topology change. This newly identified function of MCM to change DNA topology may imply a wider functional role for MCM in DNA metabolisms beyond helicase function. Finally, using yeast genetics, we show that the inter-subunit interactions, important for MCM filament formation, play a role for cell growth and survival.

PubMed: 23361460
DOI: 10.1093/nar/gkt022

実験手法

X-RAY DIFFRACTION (4.1 Å)