4FC8

Crystal structure of transcription regulator protein Rtr1 from Kluyveromyces lactis

Summary for 4FC8

• Entry DOI: 10.2210/pdb4fc8/pdb
• Descriptor: transcription protein Rtr1, ZINC ION (3 entities in total)
• Functional Keywords: zinc finger, transcription
• Biological source: Kluyveromyces lactis (yeast)
• Total number of polymer chains: 2
• Total formula weight: 51691.45

Authors

Xiang, K.,Tong, L. (deposition date: 2012-05-24, release date: 2012-07-25, Last modification date: 2024-02-28)

Primary citation

Xiang, K.,Manley, J.L.,Tong, L.
The yeast regulator of transcription protein Rtr1 lacks an active site and phosphatase activity.
Nat Commun, 3:946-946, 2012

PubMed Abstract: The activity of RNA polymerase II (Pol II) is controlled in part by the phosphorylation state of the C-terminal domain (CTD) of its largest subunit. Recent reports have suggested that yeast regulator of transcription protein, Rtr1, and its human homologue RPAP2, possess Pol II CTD Ser5 phosphatase activity. Here we report the crystal structure of Kluyveromyces lactis Rtr1, which reveals a new type of zinc finger protein and does not have any close structural homologues. Importantly, the structure does not show evidence of an active site, and extensive experiments to demonstrate its CTD phosphatase activity have been unsuccessful, suggesting that Rtr1 has a non-catalytic role in CTD dephosphorylation.

PubMed: 22781759
DOI: 10.1038/ncomms1947

Experimental method

X-RAY DIFFRACTION (2.5 Å)