4FC8

Crystal structure of transcription regulator protein Rtr1 from Kluyveromyces lactis

4FC8 の概要

• エントリーDOI: 10.2210/pdb4fc8/pdb
• 分子名称: transcription protein Rtr1, ZINC ION (3 entities in total)
• 機能のキーワード: zinc finger, transcription
• 由来する生物種: Kluyveromyces lactis (yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51691.45

構造登録者

Xiang, K.,Tong, L. (登録日: 2012-05-24, 公開日: 2012-07-25, 最終更新日: 2024-02-28)

主引用文献

Xiang, K.,Manley, J.L.,Tong, L.
The yeast regulator of transcription protein Rtr1 lacks an active site and phosphatase activity.
Nat Commun, 3:946-946, 2012

PubMed Abstract: The activity of RNA polymerase II (Pol II) is controlled in part by the phosphorylation state of the C-terminal domain (CTD) of its largest subunit. Recent reports have suggested that yeast regulator of transcription protein, Rtr1, and its human homologue RPAP2, possess Pol II CTD Ser5 phosphatase activity. Here we report the crystal structure of Kluyveromyces lactis Rtr1, which reveals a new type of zinc finger protein and does not have any close structural homologues. Importantly, the structure does not show evidence of an active site, and extensive experiments to demonstrate its CTD phosphatase activity have been unsuccessful, suggesting that Rtr1 has a non-catalytic role in CTD dephosphorylation.

PubMed: 22781759
DOI: 10.1038/ncomms1947

実験手法

X-RAY DIFFRACTION (2.5 Å)