4FBV

Crystal structure of the Myxococcus Xanthus hemagglutinin in complex with a3,a6-mannopentaose

4FBV の概要

• エントリーDOI: 10.2210/pdb4fbv/pdb
• 関連するPDBエントリー: 3OBL 3S5V 4FBO 4FBR
• 分子名称: Myxobacterial hemagglutinin, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose, ... (6 entities in total)
• 機能のキーワード: beta-barrel, hiv-inactivating, carbohydrate binding protein
• 由来する生物種: Myxococcus xanthus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29860.38

構造登録者

Koharudin, L.M.I.,Gronenborn, A.M. (登録日: 2012-05-23, 公開日: 2012-08-15, 最終更新日: 2023-09-13)

主引用文献

Koharudin, L.M.,Kollipara, S.,Aiken, C.,Gronenborn, A.M.
Structural Insights into the Anti-HIV Activity of the Oscillatoria agardhii Agglutinin Homolog Lectin Family.
J.Biol.Chem., 287:33796-33811, 2012

PubMed Abstract: Oscillatoria agardhii agglutinin homolog (OAAH) proteins belong to a recently discovered lectin family. All members contain a sequence repeat of ~66 amino acids, with the number of repeats varying among different family members. Apart from data for the founding member OAA, neither three-dimensional structures, information about carbohydrate binding specificities, nor antiviral activity data have been available up to now for any other members of the OAAH family. To elucidate the structural basis for the antiviral mechanism of OAAHs, we determined the crystal structures of Pseudomonas fluorescens and Myxococcus xanthus lectins. Both proteins exhibit the same fold, resembling the founding family member, OAA, with minor differences in loop conformations. Carbohydrate binding studies by NMR and x-ray structures of glycan-lectin complexes reveal that the number of sugar binding sites corresponds to the number of sequence repeats in each protein. As for OAA, tight and specific binding to α3,α6-mannopentaose was observed. All the OAAH proteins described here exhibit potent anti-HIV activity at comparable levels. Altogether, our results provide structural details of the protein-carbohydrate interaction for this novel lectin family and insights into the molecular basis of their HIV inactivation properties.

PubMed: 22865886
DOI: 10.1074/jbc.M112.388579

実験手法

X-RAY DIFFRACTION (1.76 Å)