4FBB

Structure of mutant RIP from barley seeds in complex with adenine (AMP-incubated)

4FBB の概要

• エントリーDOI: 10.2210/pdb4fbb/pdb
• 関連するPDBエントリー: 4FB9 4FBA 4FBC 4FBH
• 分子名称: Protein synthesis inhibitor I, ADENINE (3 entities in total)
• 機能のキーワード: hydrolase, ribosome inactivating protein, rrna n-glycosylase activity, ribosome
• 由来する生物種: Hordeum vulgare (barley)
• 細胞内の位置: Cytoplasm: P22244
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 119806.34

構造登録者

Lee, B.-G.,Kim, M.K.,Suh, S.W.,Song, H.K. (登録日: 2012-05-22, 公開日: 2012-10-31, 最終更新日: 2024-02-28)

主引用文献

Lee, B.G.,Kim, M.K.,Kim, B.W.,Suh, S.W.,Song, H.K.
Structures of the ribosome-inactivating protein from barley seeds reveal a unique activation mechanism.
Acta Crystallogr.,Sect.D, 68:1488-1500, 2012

PubMed Abstract: Ribosome-inactivating protein (RIP), a defence protein found in various plants, possesses different chain architectures and activation mechanisms. The RIP from barley (bRIP) is a type I RIP and has sequence features that are divergent from those of type I and type II RIPs from dicotyledonous plants and even the type III RIP from maize. This study presents the first crystal structure of an RIP from a cereal crop, barley, in free, AMP-bound and adenine-bound states. For phasing, a codon-optimized synthetic brip1 gene was used and a vector was constructed to overexpress soluble bRIP fusion proteins; such expression has been verified in a number of cases. The overall structure of bRIP shows folding similar to that observed in other RIPs but also shows significant differences in specific regions, particularly in a switch region that undergoes a structural transition between a 3(10)-helix and a loop depending on the liganded state. The switch region is in a position equivalent to that of a proteolytically susceptible and putative ribosome-binding site in type III RIPs. Thus, the bRIP structure confirms the detailed enzymatic mechanism of this N-glycosidase and reveals a novel activation mechanism for type I RIPs from cereal crops.

PubMed: 23090398
DOI: 10.1107/S0907444912037110

実験手法

X-RAY DIFFRACTION (1.8 Å)