4F85

Structure analysis of Geranyl diphosphate methyltransferase

4F85 の概要

• エントリーDOI: 10.2210/pdb4f85/pdb
• 関連するPDBエントリー: 4F84 4F86
• 分子名称: Geranyl diphosphate 2-C-methyltransferase (2 entities in total)
• 機能のキーワード: alpha/beta, rossmann fold, methyltransferase, transferase
• 由来する生物種: Streptomyces lasaliensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35092.07

構造登録者

Ariyawutthiphan, O.,Ose, T.,Minami, A.,Gao, Y.G.,Yao, M.,Oikawa, H.,Tanaka, I. (登録日: 2012-05-17, 公開日: 2012-10-31, 最終更新日: 2023-11-08)

主引用文献

Ariyawutthiphan, O.,Ose, T.,Minami, A.,Sinde, S.,Tsuda, M.,Gao, Y.-G.,Yao, M.,Oikawa, H.,Tanaka, I.
Structure analysis of geranyl pyrophosphate methyltransferase and the proposed reaction mechanism of SAM-dependent C-methylation
Acta Crystallogr.,Sect.D, 68:1558-1569, 2012

PubMed Abstract: In the typical isoprenoid-biosynthesis pathway, condensation of the universal C(5)-unit precursors isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) occurs via the common intermediates prenyl pyrophosphates (C(10)-C(20)). The diversity of isoprenoids reflects differences in chain length, cyclization and further additional modification after cyclization. In contrast, the biosynthesis of 2-methylisonorneol (2-MIB), which is responsible for taste and odour problems in drinking water, is unique in that it primes the enzymatic methylation of geranyl pyrophosphate (GPP) before cyclization, which is catalyzed by an S-adenosyl-L-methionine-dependent methyltransferase (GPPMT). The substrate of GPPMT contains a nonconjugated olefin and the reaction mechanism is expected to be similar to that of the steroid methyltransferase (SMT) family. Here, structural analysis of GPPMT in complex with its cofactor and substrate revealed the mechanisms of substrate recognition and possible enzymatic reaction. Using the structures of these complexes, methyl-group transfer and the subsequent proton-abstraction mechanism are discussed. GPPMT and SMTs contain a conserved glutamate residue that is likely to play a role as a general base. Comparison with the reaction mechanism of the mycolic acid cyclopropane synthase (MACS) family also supports this result. This enzyme represented here is the first model of the enzymatic C-methylation of a nonconjugated olefin in the isoprenoid-biosynthesis pathway. In addition, an elaborate system to avoid methylation of incorrect substrates is proposed.

PubMed: 23090405
DOI: 10.1107/S0907444912038486

実験手法

X-RAY DIFFRACTION (2.2 Å)