4F3M

Crystal structure of CRISPR-associated protein

Summary for 4F3M

• Entry DOI: 10.2210/pdb4f3m/pdb
• Descriptor: BH0337 protein, 1,2-ETHANEDIOL, SULFATE ION, ... (4 entities in total)
• Functional Keywords: crispr, ferredoxin fold, endoribonuclease, rna, rna binding protein
• Biological source: Bacillus halodurans
• Total number of polymer chains: 2
• Total formula weight: 54711.70

Authors

Ke, A.,Nam, K.H. (deposition date: 2012-05-09, release date: 2012-08-15, Last modification date: 2024-02-28)

Primary citation

Nam, K.H.,Haitjema, C.,Liu, X.,Ding, F.,Wang, H.,Delisa, M.P.,Ke, A.
Cas5d Protein Processes Pre-crRNA and Assembles into a Cascade-like Interference Complex in Subtype I-C/Dvulg CRISPR-Cas System.
Structure, 20:1574-1584, 2012

PubMed Abstract: Clustered regularly interspaced short palindromic repeats (CRISPRs), together with an operon of CRISPR-associated (Cas) proteins, form an RNA-based prokaryotic immune system against exogenous genetic elements. Cas5 family proteins are found in several type I CRISPR-Cas systems. Here, we report the molecular function of subtype I-C/Dvulg Cas5d from Bacillus halodurans. We show that Cas5d cleaves pre-crRNA into unit length by recognizing both the hairpin structure and the 3' single stranded sequence in the CRISPR repeat region. Cas5d structure reveals a ferredoxin domain-based architecture and a catalytic triad formed by Y46, K116, and H117 residues. We further show that after pre-crRNA processing, Cas5d assembles with crRNA, Csd1, and Csd2 proteins to form a multi-sub-unit interference complex similar to Escherichia coli Cascade (CRISPR-associated complex for antiviral defense) in architecture. Our results suggest that formation of a crRNA-presenting Cascade-like complex is likely a common theme among type I CRISPR subtypes.

PubMed: 22841292
DOI: 10.1016/j.str.2012.06.016

Experimental method

X-RAY DIFFRACTION (1.71 Å)