4F14

Structure of the SH3 domain of human nebulette in complex with a peptide of XIRP2

4F14 の概要

• エントリーDOI: 10.2210/pdb4f14/pdb
• 分子名称: Nebulette, Xin actin-binding repeat-containing protein 2, ZINC ION, ... (4 entities in total)
• 機能のキーワード: sh3 domain, heart muscle, actin-binding protein-peptide complex, actin-binding protein/peptide
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Isoform 2: Cytoplasm : O76041; Cell junction : A4UGR9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8873.28

構造登録者

Sauer, F.,Vahokoski, J.,Wilmanns, M. (登録日: 2012-05-06, 公開日: 2012-06-20, 最終更新日: 2024-02-28)

主引用文献

Eulitz, S.,Sauer, F.,Pelissier, M.C.,Boisguerin, P.,Molt, S.,Schuld, J.,Orfanos, Z.,Kley, R.A.,Volkmer, R.,Wilmanns, M.,Kirfel, G.,van der Ven, P.F.,Furst, D.O.
Identification of Xin-repeat proteins as novel ligands of the SH3 domains of nebulin and nebulette and analysis of their interaction during myofibril formation and remodeling.
Mol Biol Cell, 24:3215-3226, 2013

PubMed Abstract: The Xin actin-binding repeat-containing proteins Xin and XIRP2 are exclusively expressed in striated muscle cells, where they are believed to play an important role in development. In adult muscle, both proteins are concentrated at attachment sites of myofibrils to the membrane. In contrast, during development they are localized to immature myofibrils together with their binding partner, filamin C, indicating an involvement of both proteins in myofibril assembly. We identify the SH3 domains of nebulin and nebulette as novel ligands of proline-rich regions of Xin and XIRP2. Precise binding motifs are mapped and shown to bind both SH3 domains with micromolar affinity. Cocrystallization of the nebulette SH3 domain with the interacting XIRP2 peptide PPPTLPKPKLPKH reveals selective interactions that conform to class II SH3 domain-binding peptides. Bimolecular fluorescence complementation experiments in cultured muscle cells indicate a temporally restricted interaction of Xin-repeat proteins with nebulin/nebulette during early stages of myofibril development that is lost upon further maturation. In mature myofibrils, this interaction is limited to longitudinally oriented structures associated with myofibril development and remodeling. These data provide new insights into the role of Xin actin-binding repeat-containing proteins (together with their interaction partners) in myofibril assembly and after muscle damage.

PubMed: 23985323
DOI: 10.1091/mbc.E13-04-0202

実験手法

X-RAY DIFFRACTION (1.2 Å)