4EXW

The structure of DdrB from Deinococcus: a new fold for single-stranded DNA binding proteins.

「3KDV」から置き換えられました

4EXW の概要

• エントリーDOI: 10.2210/pdb4exw/pdb
• 分子名称: Single-stranded DNA-binding protein DdrB (2 entities in total)
• 機能のキーワード: anti-parallel beta-barrel, pentamer, dna binding, dna annealing, dna binding protein
• 由来する生物種: Deinococcus geothermalis DSM 11300
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 103338.18

構造登録者

Sugiman-Marangos, S.,Junop, M.S. (登録日: 2012-05-01, 公開日: 2012-07-11, 最終更新日: 2024-11-06)

主引用文献

Sugiman-Marangos, S.,Junop, M.S.
The structure of DdrB from Deinococcus: a new fold for single-stranded DNA binding proteins.
Nucleic Acids Res., 38:3432-3440, 2010

PubMed Abstract: Deinococcus spp. are renowned for their amazing ability to recover rapidly from severe genomic fragmentation as a result of exposure to extreme levels of ionizing radiation or desiccation. Despite having been originally characterized over 50 years ago, the mechanism underlying this remarkable repair process is still poorly understood. Here, we report the 2.8 A structure of DdrB, a single-stranded DNA (ssDNA) binding protein unique to Deinococcus spp. that is crucial for recovery following DNA damage. DdrB forms a pentameric ring capable of binding single-stranded but not double-stranded DNA. Unexpectedly, the crystal structure reveals that DdrB comprises a novel fold that is structurally and topologically distinct from all other single-stranded binding (SSB) proteins characterized to date. The need for a unique ssDNA binding function in response to severe damage, suggests a distinct role for DdrB which may encompass not only standard SSB protein function in protection of ssDNA, but also more specialized roles in protein recruitment or DNA architecture maintenance. Possible mechanisms of DdrB action in damage recovery are discussed.

PubMed: 20129942
DOI: 10.1093/nar/gkq036

実験手法

X-RAY DIFFRACTION (2.8 Å)