4EWC

Crystal Structure of the Infectious Salmon Anemia Virus Nucleoprotein

Summary for 4EWC

• Entry DOI: 10.2210/pdb4ewc/pdb
• Descriptor: Putative nucleoprotein (2 entities in total)
• Functional Keywords: alpha-helical, rna encapsidation, isavirus, orthomyoxovirus, nucleoprotein, np, rna binding, replication, transcription, viral rna packaging, rna binding protein
• Biological source: Infectious salmon anemia virus
• Total number of polymer chains: 1
• Total formula weight: 68313.72

Authors

Zheng, W.,Tao, Y.J. (deposition date: 2012-04-26, release date: 2013-05-08, Last modification date: 2024-10-16)

Primary citation

Zheng, W.,Olson, J.,Vakharia, V.,Tao, Y.J.
The crystal structure and RNA-binding of an orthomyxovirus nucleoprotein.
Plos Pathog., 9:e1003624-e1003624, 2013

PubMed Abstract: Genome packaging for viruses with segmented genomes is often a complex problem. This is particularly true for influenza viruses and other orthomyxoviruses, whose genome consists of multiple negative-sense RNAs encapsidated as ribonucleoprotein (RNP) complexes. To better understand the structural features of orthomyxovirus RNPs that allow them to be packaged, we determined the crystal structure of the nucleoprotein (NP) of a fish orthomyxovirus, the infectious salmon anemia virus (ISAV) (genus Isavirus). As the major protein component of the RNPs, ISAV-NP possesses a bi-lobular structure similar to the influenza virus NP. Because both RNA-free and RNA-bound ISAV NP forms stable dimers in solution, we were able to measure the NP RNA binding affinity as well as the stoichiometry using recombinant proteins and synthetic oligos. Our RNA binding analysis revealed that each ISAV-NP binds ~12 nts of RNA, shorter than the 24-28 nts originally estimated for the influenza A virus NP based on population average. The 12-nt stoichiometry was further confirmed by results from electron microscopy and dynamic light scattering. Considering that RNPs of ISAV and the influenza viruses have similar morphologies and dimensions, our findings suggest that NP-free RNA may exist on orthomyxovirus RNPs, and selective RNP packaging may be accomplished through direct RNA-RNA interactions.

PubMed: 24068932
DOI: 10.1371/journal.ppat.1003624

Experimental method

X-RAY DIFFRACTION (2.7 Å)