4EWC

Crystal Structure of the Infectious Salmon Anemia Virus Nucleoprotein

4EWC の概要

• エントリーDOI: 10.2210/pdb4ewc/pdb
• 分子名称: Putative nucleoprotein (2 entities in total)
• 機能のキーワード: alpha-helical, rna encapsidation, isavirus, orthomyoxovirus, nucleoprotein, np, rna binding, replication, transcription, viral rna packaging, rna binding protein
• 由来する生物種: Infectious salmon anemia virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 68313.72

構造登録者

Zheng, W.,Tao, Y.J. (登録日: 2012-04-26, 公開日: 2013-05-08, 最終更新日: 2024-10-16)

主引用文献

Zheng, W.,Olson, J.,Vakharia, V.,Tao, Y.J.
The crystal structure and RNA-binding of an orthomyxovirus nucleoprotein.
Plos Pathog., 9:e1003624-e1003624, 2013

PubMed Abstract: Genome packaging for viruses with segmented genomes is often a complex problem. This is particularly true for influenza viruses and other orthomyxoviruses, whose genome consists of multiple negative-sense RNAs encapsidated as ribonucleoprotein (RNP) complexes. To better understand the structural features of orthomyxovirus RNPs that allow them to be packaged, we determined the crystal structure of the nucleoprotein (NP) of a fish orthomyxovirus, the infectious salmon anemia virus (ISAV) (genus Isavirus). As the major protein component of the RNPs, ISAV-NP possesses a bi-lobular structure similar to the influenza virus NP. Because both RNA-free and RNA-bound ISAV NP forms stable dimers in solution, we were able to measure the NP RNA binding affinity as well as the stoichiometry using recombinant proteins and synthetic oligos. Our RNA binding analysis revealed that each ISAV-NP binds ~12 nts of RNA, shorter than the 24-28 nts originally estimated for the influenza A virus NP based on population average. The 12-nt stoichiometry was further confirmed by results from electron microscopy and dynamic light scattering. Considering that RNPs of ISAV and the influenza viruses have similar morphologies and dimensions, our findings suggest that NP-free RNA may exist on orthomyxovirus RNPs, and selective RNP packaging may be accomplished through direct RNA-RNA interactions.

PubMed: 24068932
DOI: 10.1371/journal.ppat.1003624

実験手法

X-RAY DIFFRACTION (2.7 Å)