4EU1

Structure of a mitochondrial aspartate aminotransferase from Trypanosoma brucei

4EU1 の概要

• エントリーDOI: 10.2210/pdb4eu1/pdb
• 分子名称: Mitochondrial aspartate aminotransferase, 1,2-ETHANEDIOL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: ssgcid, aspartate aminotransferase, structural genomics, seattle structural genomics center for infectious disease, pyridoxalphosphate lysine, mitochondrial, transferase
• 由来する生物種: Trypanosoma brucei
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91144.91

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2012-04-25, 公開日: 2012-05-16, 最終更新日: 2023-12-06)

主引用文献

Abendroth, J.,Choi, R.,Wall, A.,Clifton, M.C.,Lukacs, C.M.,Staker, B.L.,Van Voorhis, W.,Myler, P.,Lorimer, D.D.,Edwards, T.E.
Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia.
Acta Crystallogr F Struct Biol Commun, 71:566-571, 2015

PubMed Abstract: The structures of three aspartate aminotransferases (AATs) from eukaryotic pathogens were solved within the Seattle Structural Genomics Center for Infectious Disease (SSGCID). Both the open and closed conformations of AAT were observed. Pyridoxal phosphate was bound to the active site via a Schiff base to a conserved lysine. An active-site mutant showed that Trypanosoma brucei AAT still binds pyridoxal phosphate even in the absence of the tethering lysine. The structures highlight the challenges for the structure-based design of inhibitors targeting the active site, while showing options for inhibitor design targeting the N-terminal arm.

PubMed: 25945710
DOI: 10.1107/S2053230X15001831

実験手法

X-RAY DIFFRACTION (2.3 Å)