4EQ6

The crystal structure of Psy3-Csm2 complex from budding yeast

Summary for 4EQ6

• Entry DOI: 10.2210/pdb4eq6/pdb
• Descriptor: Chromosome segregation in meiosis protein 2, Platinum sensitivity protein 3 (3 entities in total)
• Functional Keywords: dna binding protein
• Biological source: Saccharomyces cerevisiae (yeast); More
• Cellular location: Cytoplasm: P40465; Nucleus: Q12318
• Total number of polymer chains: 2
• Total formula weight: 54959.31

Authors

She, Z.,Gao, Z.Q.,Dong, Y.H. (deposition date: 2012-04-18, release date: 2012-08-01, Last modification date: 2024-03-20)

Primary citation

She, Z.,Gao, Z.Q.,Liu, Y.,Wang, W.J.,Liu, G.F.,Shtykova, E.V.,Xu, J.H.,Dong, Y.H.
Structural and SAXS analysis of the budding yeast SHU-complex proteins
FEBS Lett., 586:2306-2312, 2012

PubMed Abstract: In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å resolution and successfully fitted into our small angle X-ray scattering (SAXS) data of the SHU-complex. Taken together with our electrophoretic mobility shift assay (EMSA) results, a model is proposed for the SHU-protein complex coupled with DNA.

PubMed: 22749910
DOI: 10.1016/j.febslet.2012.06.024

Experimental method

X-RAY DIFFRACTION (1.8 Å)