4EQ6

The crystal structure of Psy3-Csm2 complex from budding yeast

4EQ6 の概要

• エントリーDOI: 10.2210/pdb4eq6/pdb
• 分子名称: Chromosome segregation in meiosis protein 2, Platinum sensitivity protein 3 (3 entities in total)
• 機能のキーワード: dna binding protein
• 由来する生物種: Saccharomyces cerevisiae (yeast); 詳細
• 細胞内の位置: Cytoplasm: P40465; Nucleus: Q12318
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54959.31

構造登録者

She, Z.,Gao, Z.Q.,Dong, Y.H. (登録日: 2012-04-18, 公開日: 2012-08-01, 最終更新日: 2024-03-20)

主引用文献

She, Z.,Gao, Z.Q.,Liu, Y.,Wang, W.J.,Liu, G.F.,Shtykova, E.V.,Xu, J.H.,Dong, Y.H.
Structural and SAXS analysis of the budding yeast SHU-complex proteins
FEBS Lett., 586:2306-2312, 2012

PubMed Abstract: In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å resolution and successfully fitted into our small angle X-ray scattering (SAXS) data of the SHU-complex. Taken together with our electrophoretic mobility shift assay (EMSA) results, a model is proposed for the SHU-protein complex coupled with DNA.

PubMed: 22749910
DOI: 10.1016/j.febslet.2012.06.024

実験手法

X-RAY DIFFRACTION (1.8 Å)