4EP4

Thermus thermophilus RuvC structure

4EP4 の概要

• エントリーDOI: 10.2210/pdb4ep4/pdb
• 関連するPDBエントリー: 4EP5
• 分子名称: Crossover junction endodeoxyribonuclease RuvC, MAGNESIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: resolvase, hydrolase
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35680.01

構造登録者

Chen, L.,Shi, K.,Yin, Z.Q.,Aihara, H. (登録日: 2012-04-17, 公開日: 2012-11-14, 最終更新日: 2024-02-28)

主引用文献

Chen, L.,Shi, K.,Yin, Z.,Aihara, H.
Structural asymmetry in the Thermus thermophilus RuvC dimer suggests a basis for sequential strand cleavages during Holliday junction resolution.
Nucleic Acids Res., 41:648-656, 2013

PubMed Abstract: Holliday junction (HJ) resolvases are structure-specific endonucleases that cleave four-way DNA junctions (HJs) generated during DNA recombination and repair. Bacterial RuvC, a prototypical HJ resolvase, functions as homodimer and nicks DNA strands precisely across the junction point. To gain insights into the mechanisms underlying symmetrical strand cleavages by RuvC, we performed crystallographic and biochemical analyses of RuvC from Thermus thermophilus (T.th. RuvC). The crystal structure of T.th. RuvC shows an overall protein fold similar to that of Escherichia coli RuvC, but T.th. RuvC has a more tightly associated dimer interface possibly reflecting its thermostability. The binding mode of a HJ-DNA substrate can be inferred from the shape/charge complementarity between the T.th. RuvC dimer and HJ-DNA, as well as positions of sulfate ions bound on the protein surface. Unexpectedly, the structure of T.th. RuvC homodimer refined at 1.28 Å resolution shows distinct asymmetry near the dimer interface, in the region harboring catalytically important aromatic residues. The observation suggests that the T.th. RuvC homodimer interconverts between two asymmetric conformations, with alternating subunits switched on for DNA strand cleavage. This model provides a structural basis for the 'nick-counter-nick' mechanism in HJ resolution, a mode of HJ processing shared by prokaryotic and eukaryotic HJ resolvases.

PubMed: 23118486
DOI: 10.1093/nar/gks1015

実験手法

X-RAY DIFFRACTION (1.28 Å)